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利用大鼠坐骨神经钳夹损伤模型，观察了碱性成纤维细胞生长因子（ｂＦＧＦ）对大鼠脊髓前角运动神经元的保护作用。ＳＤ成年大鼠４０只，分成实验组和对照组，钳夹损伤大鼠坐骨神经。在实验组损伤侧受损的神经周围放入浸有ｂＦＧＦ的明胶海绵，对照组则用生理盐水浸泡海绵置于受损神经处。以大鼠右侧坐骨神经为正常自身空白对照。术后隔日一次向损伤侧腓肠肌肌注ｂＦＧＦ，对照组注射等量生理盐水。术后２、３、４、５周分别对每只大鼠进行心脏灌注固定，切取脊髓腰４～６节段，恒冷箱冷冻切片，Ｎｉｓｌ染色，观察脊髓前角运动元的形态，计算其数目，将损伤侧与正常侧比较，将ｂＦＧＦ治疗组与非治疗组作比较，统计学处理。结果：术后３、４、５周治疗组的损伤侧脊髓前角运动神经元存活率均大于对照组，且统计差异有显著性意义（Ｐ＜０．０５）。表明ｂＦＧＦ对坐骨神经钳夹损伤所导致的脊髓前角运动神经元胞体死亡有保护作用，也许可能成为运动神经元新的神经营养因子。
The rat sciatic nerve clamp injury model was used to observe the protective effect of basic fibroblast growth factor (bFGF) on anterior horn motor neurons in the spinal cord of rats. Forty SD adult rats were divided into experimental group and control group, and the injured rats were sciatic nerves. Gelatin sponge soaked with bFGF was placed around the damaged nerve in the experimental group, while the control group was immersed in saline with saline soaked sponge. Right rat sciatic nerve as the normal self-control. The bFGF was intramuscularly injected into injured gastrocnemius muscle on the next day, and the control group was injected with the same amount of saline. At 2, 3, 4, 5 weeks after operation, each rat was perfused with heart. Four to six segments of the lumbar spinal cord were excised. Frozen sections were fixed with a cryostat and stained with Nisl. Morphology of anterior horn of spinal cord was observed. The number of injury side compared with the normal side, bFGF treatment group and non-treatment group for comparison, statistical analysis. Results: After 3, 4, and 5 weeks of operation, the survival rate of injured spinal cord anterior horn motoneurons were all higher than that of control group, and the difference was statistically significant (P <0.05). These results suggest that bFGF may have a protective effect on the death of motor neurons in the anterior horn of spinal cord induced by sciatic nerve clamp injury and may be the new neurotrophic factor of motor neurons.