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为了研究蝴蝶兰SOC1基因的功能,为蝴蝶兰分子育种等方面的研究提供一定的理论依据。本研究以蝴蝶兰“聚宝”盛花期的花瓣为材料提取总RNA,采用RT-PCR方法克隆蝴蝶兰SOC1基因的编码区序列,片段长度为682 bp,共编码219个氨基酸,该基因命名为SOC1,登录号为:KT852838。对蝴蝶兰SOC1基因进行同源分析及构建系统发育树,分析结果显示,与SOC1核苷酸序列同源性最高的是小兰屿蝴蝶兰(Phalaenopsis equestris),达到93.72%,SOC1基因与兰科植物小兰屿蝴蝶兰及石斛的亲缘关系最近。实时荧光定量PCR分析表明,SOC1基因在的不同时期不同部位都有表达,但表达丰度不一致。在根中,营养生长期表达量最高;在叶中,抽葶期表达水平最高;在花葶中,抽葶期、开花期和子房发育期的表达水平相当;在花器官中,以蕊柱的表达量最高,其次是子房,而花萼、唇瓣、侧瓣中只有微量表达。研究认为SOC1基因在蝴蝶兰生长发育中既调控营养生长,又调控生殖生长;在花器官中主要参与蕊柱和子房的发育。
In order to study the function of Phalaenopsis SOC1 gene and to provide a theoretical basis for researches on the molecular breeding of Phalaenopsis. In this study, the total RNA was extracted from the petals of Phalaenopsis orchard. The sequence of the coding region of Phalaenopsis SOC1 was cloned by RT-PCR. The fragment was 682 bp in length encoding a total of 219 amino acids. The gene was named For SOC1, accession number: KT852838. Homology analysis of Phalaenopsis SOC1 gene and phylogenetic tree analysis showed that Phalaenopsis equestris with the highest homology with SOC1 nucleotide sequence was 93.72%, SOC1 gene was closely related to Orchidaceae Plant Portland orchid Phalaenopsis and the closest genetic relationship. Real-time PCR analysis showed that SOC1 gene was expressed in different sites at different stages, but the expression abundance was inconsistent. In roots, the highest expression was in vegetative stage; in leaves, the expression level was the highest at pumping stage; in flowering stage, the expression level in pumping stage, flowering stage and ovary development stage were quite equal; in flower organs, Of the highest expression, followed by the ovary, and calyx, lip, side flap only a small amount of expression. The study suggests that SOC1 gene not only regulates vegetative growth but also regulates reproductive growth in the growth and development of Phalaenopsis, and mainly participates in the development of stamen and ovary in floral organs.