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目的:观察复方木鸡颗粒对小鼠慢性肝损伤的作用,初步探讨其可能的作用机制。方法:采用高脂饲料、CCl4、D-氨基半乳糖盐酸盐、刀豆蛋白(ConA)建立4种慢性肝损伤模型,测定小鼠血清转氨酶(ALT、AST)水平、肝指数和肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的水平,并做肝组织病理学检查。另高脂饲料所致的慢性肝损伤检测肝组织中三酰甘油(TG)含量,ConA所致的免疫性肝损伤取脾脏,称重,计算脾指数。结果:复方木鸡颗粒能显著降低4种慢性肝损伤小鼠的肝指数以及血清中AST与ALT(P<0.05或P<0.01)和肝组织中MDA水平(P<0.05或P<0.01),升高SOD水平(P<0.05或P<0.01)。高脂饲料所致慢性肝损伤小鼠肝组织中的TG含量显著降低(P<0.05或P<0.01),免疫性肝损伤中脾指数亦减小(P<0.05或P<0.01)。结论:复方木鸡颗粒对4种慢性肝损伤有不同程度的防治作用,其作用机制可能与抑制炎症因子的释放、抑制脂质过氧化反应、清除自由基等有关。
Objective: To observe the effect of compound wood chicken on chronic liver injury in mice, and to explore its possible mechanism. Methods: Four kinds of chronic liver injury models were established by high-fat diet, CCl4, D-galactosamine hydrochloride and ConA. Serum transaminase (ALT, AST), liver index Malondialdehyde (MDA), superoxide dismutase (SOD) levels, and do the liver histopathological examination. Another high-fat diet-induced chronic liver injury in liver tissue triglyceride (TG) content, ConA induced immune liver injury to take the spleen, weighing, calculate the spleen index. Results: Fujiwagi Granules could significantly reduce the liver index and serum AST and ALT (P <0.05 or P <0.01) and MDA levels (P <0.05 or P <0.01) in four kinds of chronic liver injury mice, Increase SOD level (P <0.05 or P <0.01). The content of TG in the liver tissue of mice with chronic liver injury induced by high-fat diet was significantly lower (P <0.05 or P <0.01), and the spleen index of immune liver injury was also decreased (P <0.05 or P <0.01). CONCLUSION: Fujiwagi granules can prevent and treat 4 kinds of chronic liver injury in varying degrees. Its mechanism may be related to the inhibition of the release of inflammatory factors, the inhibition of lipid peroxidation and the elimination of free radicals.