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目的研究生物活性肽Exenatide对2型糖尿病胰岛素抵抗模型大鼠组织JAK1/STAT1的表达及胰岛B细胞凋亡的影响。方法用低剂量链脲佐菌素(STZ)联合高脂喂养的方法建立胰岛素抵抗大鼠模型,分别用Exenatide、二甲双胍和生理盐水作用于模型大鼠。检测大鼠糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)、空腹血糖(FBG)等生化指标、计算胰岛素敏感指数(ISI),观察Exenatide对胰岛素抵抗模型大鼠的疗效。蛋白质印迹法检测大鼠胰岛组织JAK1转录激活子1(STAT1)的蛋白表达水平;Annexin-Ⅴ/PI染色法比较各组胰岛B细胞对氧化应激诱发的细胞凋亡率。结果与生理盐水对照组相比,Exenatide治疗组ISI增高,糖化血红蛋白降低(P均<0.05)。与生理盐水对照组和二甲双胍治疗组相比,Exenatide组胰岛组织中JAK1/STAT1蛋白表达水平降低(P<0.01),且H2O2诱发的胰岛B细胞凋亡率降低(P<0.01)。结论 Exenatide可能通过调节JAK1/STAT1的表达,改善胰岛素抵抗,抑制B细胞凋亡。
Objective To study the effect of Exenatide on JAK1 / STAT1 expression and islet B cell apoptosis in type 2 diabetic rats with insulin resistance. Methods Insulin resistant rat models were established by low-dose streptozotocin (STZ) combined with high-fat diet. Exenatide, metformin and saline were used respectively to induce model rats. The biochemical indexes such as HbA1c, FINS and FBG in rats were measured, and insulin sensitivity index (ISI) was calculated to observe the effect of Exenatide on insulin resistance model rats. Western blotting was used to detect the protein expression of JAK1 transcriptional activator 1 (STAT1) in rat pancreatic islets. Annexin-Ⅴ / PI staining was used to compare the apoptotic rate induced by oxidative stress. Results Compared with the saline control group, the ISI of the Exenatide treatment group was increased and the HbA1c was decreased (all P <0.05). Compared with saline control group and metformin treatment group, the expression of JAK1 / STAT1 protein in pancreatic islets decreased (P <0.01) and the apoptosis rate of islet B cells induced by H2O2 decreased (P <0.01). Conclusion Exenatide may improve insulin resistance and inhibit B cell apoptosis by regulating the expression of JAK1 / STAT1.