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Effects of titanium oxide (TiO 2) nanoparticles on Bel-7402 human hepatoma cells and L-02 human hepatocytes at different times were observed.Using cell culture,cell growth curves of Bel-7402 cells and L-02 cells treated with TiO 2 nanoparticles were examined by MTT assay,and the cellular ultrastructure was observed by an analytical transmission electron microscope (ATEM).It is found that OD value of Bel-7402 cell treated with TiO 2 nanoparticles for 48-144h is obviously lower than that of control group (p<0.01).However the growth curve of L-02 cells is almost not affected by TiO 2 nanoparticles.ATEM and energy dispersive X ray (EDX) analyses show that there are obvious vacuoles increased heterolysosome,and particles with high electron density which are confirmed to be TiO 2 nanoparticles in Bel-7402 cytoplasm.More interestingly,it is alse found that TiO 2 nanoparticle obviously inhibits the proliferation of hepatoma cells by altering lysosome activity and destroying cytoplasm structure.The inhibition on proliferation of hepatocytes by TiO 2 nanoparticles is much slighter.The results demonstrate that TiO 2 nanoparticle has different killing effects on cancer cell and normal cell.
Effects of titanium oxide (TiO 2) nanoparticles on Bel-7402 human hepatoma cells and L-02 human hepatocytes at different times were observed. Using cell culture, cell growth curves of Bel-7402 cells and L-02 cells treated with TiO 2 nanoparticles were examined by MTT assay, and the cellular ultrastructure was observed by an analytical transmission electron microscope (ATEM). It is found that OD value of Bel-7402 cell treated with TiO 2 nanoparticles for 48-144h is obviously lower than that of control group (p <0.01) .Wowever the growth curve of L-02 cells is almost not affected by TiO 2 nanoparticles. TEM and energy dispersive X ray (EDX) analyzes show that there are obvious vacuoles increased heterolysosomes, and particles with high electron density which are confirmed to be TiO 2 nanoparticles in Bel-7402 cytoplasm. More interestingly, it is alse found that TiO 2 nanoparticle obviously inhibits the proliferation of hepatoma cells by altering lysosome activity and destroying cytoplasm st ructure. The inhibition on proliferation of hepatocytes by TiO 2 nanoparticles is much slighter. The results demonstrate that TiO 2 nanoparticle has different killing effects on cancer cells and normal cells.