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探讨虫草多糖CPS-A对AngⅡ诱导的人肝L02细胞损伤的保护作用。MTT法考察AngⅡ、CPS-A对L02细胞增殖的影响,AngⅡ刺激L02细胞后,考察CPS-A对L02细胞的损伤保护作用;PCR、Real-Time PCR和Western blot法检测IL-6、IL-1β、AT1R、AT2R、NF-κB p65、TNFα等因子mRNA和蛋白质的表达水平。结果表明:AngⅡ、CPS-A有效抑制L02细胞增殖的浓度分别为1×10~(-5)mol/L和200μg/m L,PCR、Real-Time PCR和Western blot结果显示,CPS-A可以显著下调IL-6、IL-1β、TNF-α、NF-κB和AT1R的表达。CPS-A对AngⅡ诱导的L02细胞损伤具有很好的保护作用。
To investigate the protective effect of Cordyceps polysaccharide CPS-A on AngⅡ-induced L02 cell injury in human liver. The effect of CPS-A on the proliferation of L02 cells was examined by MTT assay and the effect of CPS-A on the proliferation of L02 cells. Angiotensin II (AngⅡ) 1β, AT1R, AT2R, NF-κB p65, TNFα and other factors mRNA and protein expression levels. The results showed that Ang Ⅱ and CPS-A effectively inhibited the proliferation of L02 cells at concentrations of 1 × 10 -5 mol / L and 200 μg / mL, respectively. PCR, Real-Time PCR and Western blot showed that CPS-A Significantly downregulated the expression of IL-6, IL-1β, TNF-α, NF-κB and AT1R. CPS-A has a good protective effect on AngⅡ-induced L02 cell injury.