论文部分内容阅读
[Objective] This study aimed to investigate the major contributors to the measurement uncertainty in quantitative analysis of genetically modified ingredients and improve the quality of quantitative detection of genetically modified components. [Method] The content of Ca MV35 S promoter( parameter) in GTS40-3-2 soybean powder samples was measured to estimate the measurement uncertainty preliminarily. [Result]Type A uncertainty( uA),type B uncertainty(uB) and combined standard uncertainty( uC) were 0. 0 004,0. 002 and 0. 002,respectively. At a confidence level of p = 95% and freedom degree of Veff= 3 251,coverage factor k = 1. 96,expanded uncertainty U = 0. 004. The final measurement result was C = 0. 028 ± 0. 004,which was close to the conventional true value(0. 03).Thus,the measurement uncertainty was relatively small,indicating a high quality of measurement. In this study,uncertainty evaluation indicated that the deviation of micro liquid transfer made the greatest contribution to the measurement uncertainty. [Cluclusion]The deviation of micro liquid transfer should be reduced to improve the quality of measurement.
[Objective] This study aims to investigate the major contributors to the measurement uncertainty in quantitative analysis of genetically modified ingredients and improve the quality of quantitative detection of genetically modified components. [Method] The content of Ca MV35 S promoter (parameter) in GTS40- [Result] Type A uncertainty (uA), type B uncertainty (uB) and combined standard uncertainty (uC) were 0. 0 004,0. 002 and 0. 002, respectively. At a confidence level of p = 95% and freedom degree of Veff = 3 251, coverage factor k = 1.96, expanded uncertainty U = 0. 004. The final measurement result was C = 0. 028 ± 0 004, which was close to the conventional true value (0. 03). Thus, the measurement uncertainty was relatively small, indicating a high quality of measurement. In this study, uncertainty evaluation indicated that the deviation of micro liquid transfer made the greatest contribution to t he measurement uncertainty. [Cluclusion] The deviation of micro liquid transfer should be reduced to improve the quality of measurement.