目的研究Ｄ５Ｓ８１８，Ｄ７Ｓ８２０的多态性及法医学应用价值。方法应用聚合酶链反应（ＰＣＲ）、聚丙烯酰胺凝胶电泳分离及银染显带技术对武汉地区汉族２３２例无关个体作Ｄ５Ｓ８１８，Ｄ７Ｓ８２０位点分型调查。结果Ｄ５Ｓ８１８和Ｄ７Ｓ８２０位点分别检出８个和６个等位基因，获汉族人群基因频率分布。二位点基因型频率分布符合ＨａｒｄｙＷｅｉｎｂｅｒｇ平衡。位点杂合度分别为０８１２１和０７９３４，个人识别能力分别为０９４１６和０９２５５，非父排除率分别为０５８４２和０５８１６。结论Ｄ５Ｓ８１８和Ｄ７Ｓ８２０ＳＴＲ位点均是高杂合度、高鉴别能力的遗传标记系统，在法医学个人识别和亲子鉴定中有较高实用价值 Objective To study the polymorphism of D5S818 and D7S820 and the forensic application value. Methods The genotypes of D5S818 and D7S820 in 232 Han unrelated individuals in Wuhan were genotyped by polymerase chain reaction (PCR), polyacrylamide gel electrophoresis and silver staining. Results Eight alleles and six alleles were detected in D5S818 and D7S820 loci, respectively. The frequency distribution of the two loci was in accordance with Hardy-Weinberg equilibrium. The heterozygosity of the loci was 08121 and 07934, respectively. The individual recognition abilities were 09416 and 09255, respectively. The non-father exclusion rates were 05842 and 05816, respectively. Conclusion Both D5S818 and D7S820STR loci are highly heterozygous and highly differentiable genetic marker systems that have high practical value in personal identification and paternity testing of forensic science