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目的 评价长期低浓度接触丙烯腈对大鼠脑单胺类神经递质及其代谢产物的影响。方法 雄性SD大鼠 30只,随机分成对照组、低剂量组和高剂量组,每组 10只。通过饮水对大鼠进行丙烯腈染毒,分别给予 0、50和 200mg/L的丙烯腈溶液。染毒时间为 12周。染毒结束后从每组随机选取 7只大鼠迅速分离双侧纹状体和小脑皮层,测定单胺类神经递质及其代谢产物的浓度,并取大脑皮层测定单胺氧化酶活性。结果 随着染毒剂量的增加,低剂量组和高剂量组大鼠纹状体中的多巴胺含量分别降低到 (2 .2±0 .7)和 (3 .2±2 .0)μg/g脑湿重,与对照组的多巴胺含量 ( 9. 0±4.2)μg/g脑湿重比较,差异有统计学意义。3组大鼠小脑中的多巴胺未见减少,其代谢产物 3, 4 双羟苯乙酸分别为(186±41)、(245±90)和(115±65)ng/g脑湿重,低剂量组显著升高。3组染毒大鼠纹状体内的 5 羟色胺含量分别为 (249±34)、(155±95)和 (128±101)ng/g脑湿重,呈逐渐下降趋势,并具有显著的剂量 效应关系,但 3组大鼠小脑中的 5- 羟色胺及其代谢产物含量的变化无统计学意义。3组大鼠脑组织纹状体和小脑中的去甲肾上腺素及其代谢产物以及脑皮层单胺氧化酶活性的改变均无统计学意义。结论 丙烯腈对单胺类神经递质及其代谢产物的影响可能是其神经行为毒性的生物学
Objective To evaluate the effects of long-term low-level exposure to acrylonitrile on monoamine neurotransmitters and their metabolites in rat brain. Methods Thirty male SD rats were randomly divided into control group, low dose group and high dose group, with 10 rats in each group. Rats were subjected to acrylonitrile exposure by drinking water, and acrylonitrile solutions of 0, 50 and 200 mg / L were respectively administered. Exposure time is 12 weeks. After the exposure, seven rats were randomly selected from each group to rapidly separate the bilateral striatum and the cerebellar cortex. The concentrations of monoamine neurotransmitters and their metabolites were measured and the monoamine oxidase activity was measured in the cerebral cortex. Results The dopamine levels in the striatum decreased to (2. 2 ± 0. 7) and (3 ± 2. 0) μg / g Brain wet weight, compared with the control group of dopamine content (9. 0 ± 4.2) g / g brain wet weight, the difference was statistically significant. Dopamine in the cerebellum of the three groups showed no decrease, and the metabolites 3, 4 of dipyridamole were (186 ± 41), (245 ± 90) and (115 ± 65) ng / g brain wet weight Group significantly increased. The content of serotonin in striatum in the 3 groups was (249 ± 34), (155 ± 95) and (128 ± 101) ng / g, respectively, showing a gradual downward trend with significant dose effects However, the content of serotonin and its metabolites in cerebellum of three groups had no significant change. Norepinephrine and its metabolites in the striatum and cerebellum, as well as changes in monoamine oxidase activity in the cortex of the 3 groups were not statistically significant. Conclusion The effects of acrylonitrile on monoamine neurotransmitters and their metabolites may be related to their neurobehavioral toxicity in biology