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目的检测类风湿性关节炎(Rheumatoid arthritis,RA)患者血清葡萄糖6磷酸异构酶(Glucose-6-phosphateisomerase,GPI)水平,研究GPI对RA的诊断价值。方法用ELISA方法检测86例RA患者、52例其它自身免疫性疾病患者和65例健康人血清中GPI和抗环瓜氨酸多肽(Anti-cyclic citrullinated peptide,抗CCP)水平。用免疫透射比浊法检测类风湿因子(RA)水平。结果 RA患者血清GPI浓度为(1.95±1.12)μg/ml,其它自身免疫性疾病组GPI浓度为(0.14±0.22)μg/ml,正常对照组GPI浓度为(0.11±0.08)μg/ml。GPI与IgM-RF之间具有相关性(r=0.568,P<0.05),但不完全重叠。GPI与抗CCP抗体之间未显示出相关性(r=0.083,P=0.515),二者也不完全重叠。结论 GPI抗原是检测RA的一项特异性很高的血清学指标,可能成为诊断RA患者的一个新的实验诊断指标。
Objective To investigate the level of serum glucose 6-phosphate isomerase (GPI) in patients with rheumatoid arthritis (RA) and to investigate the diagnostic value of GPI in RA. Methods The levels of GPI and anti-cyclic citrullinated peptide (anti-CCP) in serum of 86 patients with RA, 52 patients with other autoimmune diseases and 65 healthy controls were detected by ELISA. Rheumatoid factor (RA) levels were measured by immunoturbidimetry. Results The GPI of RA patients was (1.95 ± 1.12) μg / ml, while that of other autoimmune diseases was (0.14 ± 0.22) μg / ml and that of the normal controls was (0.11 ± 0.08) μg / ml. There was a correlation between GPI and IgM-RF (r = 0.568, P <0.05) but not complete overlap. There was no correlation between GPI and anti-CCP antibody (r = 0.083, P = 0.515), nor did they completely overlap. Conclusion GPI antigen is a highly specific serological marker for the detection of RA, which may be a new experimental diagnostic indicator for the diagnosis of RA.