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目的比较体外培养正常成年大鼠、大鼠乳鼠及人胎肝细胞的生物学性能。方法采用不同的分离方法获取肝细胞进行体外培养 ,台盼蓝染色法测定细胞活率 ,ＭＴＴ法检测细胞增殖活力 ,通过检测白蛋白分泌反映肝细胞的功能。结果人胎肝细胞生长活力和蛋白合成功能最佳 ,乳鼠肝细胞次之 ;乳鼠肝细胞较人胎肝细胞易传代 ;成年大鼠肝细胞分离操作方便 ,但在体外存活时间较短。结论不同取材来源的肝细胞体外培养性能有显著性异 (Ｐ < 0 . 0 5)。
Objective To compare the biological characteristics of normal adult rats, rat neonatal rats and human fetal hepatocytes cultured in vitro. Methods Hepatic cells were isolated by different methods. Cell viability was measured by trypan blue staining. Cell viability was measured by MTT assay. The function of hepatocytes was detected by detecting albumin secretion. Results The fetal liver cells had the best growth activity and protein synthesis, followed by the neonatal rat hepatocytes. The neonatal rat hepatocytes were more easily passaged than the human fetal hepatocytes. The isolation of adult rat hepatocytes was easy, but the survival time in vitro was short. Conclusion The hepatocytes cultured in vitro showed significant differences in vitro (P <0.05).