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目的观察刮痧对腰椎间盘突出症(LDH)模型大鼠背根神经节(DRG)中炎性细胞因子和血清疼痛物质的影响,探讨刮痧治疗LDH的作用机制。方法 30只雄性SD大鼠随机分为假手术组、模型组、刮痧组。采用自体髓核移植法建立LDH大鼠模型。刮痧组于造模后第5天开始刮痧治疗,假手术组及模型组不予干预措施。干预三个疗程后处死取材。应用免疫组化法检测大鼠背根神经节中炎性细胞因子磷脂酶A_2(PLA_2)和前列腺素E_2(PGE_2)的含量。应用酶联免疫法(ELISA)检测大鼠血清中疼痛物质P物质(SP)和神经肽Y(NPY)的含量。结果与假手术组比较,模型组背根神经节中PLA_2、PGE_2及血清SP、NPY含量均明显升高,差异有统计学意义(P<0.01);与模型组比较,刮痧组背根神经节中PLA_2、PGE_2及血清SP、NPY含量均明显降低,差异有统计学意义(P<0.01)。结论刮痧可有效抑制LDH模型大鼠背根神经节中炎症因子PLA_2、PGE_2和血清疼痛物质SP、NPY的表达,发挥抗炎和神经调节的作用。
Objective To observe the effect of scraping on inflammatory cytokines and serum pain substances in dorsal root ganglion (DRG) of rats with lumbar disc herniation (LDH), and to explore the mechanism of action of scraping on LDH. Methods Thirty male SD rats were randomly divided into sham operation group, model group and shaving group. LDH rat model was established by autologous nucleus transplantation. Scrapping group in the first 5 days after modeling began scraping treatment, sham-operated group and model group without intervention. Intervention after three courses were sacrificed. The levels of inflammatory cytokines phospholipase A_2 (PLA_2) and prostaglandin E_2 (PGE_2) in rat dorsal root ganglion were detected by immunohistochemistry. Serum contents of substance P (SP) and neuropeptide Y (NPY) were determined by enzyme-linked immunosorbent assay (ELISA). Results Compared with the sham-operation group, PLA 2, PGE 2 and SP, NPY in DRG significantly increased in the model group (P <0.01). Compared with the model group, the dorsal root ganglion PLA 2, PGE 2 and serum SP, NPY levels were significantly lower, the difference was statistically significant (P <0.01). Conclusion Scrapping can effectively inhibit the expression of inflammatory cytokines PLA_2, PGE_2 and SP, NPY in the dorsal root ganglion of LDH model rats and exert anti-inflammatory and neuromodulatory effects.