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荫蔽导致植物所受红光/远红光(R/FR)比值下降,为探究荫蔽对大豆幼苗异黄酮合成的影响,本试验以耐荫性差异显著的大豆品种“ND12”和“C103”幼苗为研究材料,分别施以不同比例红光/远红光(R/FR)处理,对其叶片、下胚轴、根系中异黄酮积累量及关键酶基因表达情况进行比较研究。结果表明,低R/FR条件下,强耐荫品种“ND12”叶片中总异黄酮及染料木素GE的含量极显著上升,下胚轴中极显著下降,弱耐荫品种“C103”则相反。同时,低R/FR使强耐荫品种“ND12”叶片中异黄酮合成关键基因IFS2、4CL、C4H和CHI的表达量极显著上调,其变化趋势与异黄酮积累变化规律一致,而弱耐荫性品种“C103”则存在较大差异。荫蔽信号诱导耐荫型大豆品种异黄酮合成,并在受胁迫部位叶片中大量积累。本研究初步揭示了荫蔽信号(低R/FR)对苗期大豆异黄酮的调控规律,为研究大豆异黄酮响应荫蔽信号的调控机理奠定了基础。
In order to explore the effect of shading on the isoflavone synthesis of soybean seedlings, the effects of shading on the ratio of red / far red light (R / FR) to plants were studied. In this experiment, soybean varieties “ND12” and “ C103 seedlings were used to study the accumulation of isoflavones and the expression of key enzyme genes in leaves, hypocotyls, and root system under different proportions of red / far red light (R / FR) treatment. The results showed that under low R / FR conditions, the content of total isoflavones and genistein GE in the highly shade-tolerant varieties ”ND12“ increased significantly, and the content of genistein in the hypocotyls significantly decreased, while that in the weak shade-tolerant varieties ”C103 “The opposite. At the same time, low R / FR significantly up-regulated the expression of IFS2, 4CL, C4H and CHI, which are the key genes for isoflavone synthesis in the strong shade tolerant ”ND12“ leaves. The trend was consistent with that of isoflavone accumulation, Shade tolerance varieties ”C103 " there is a big difference. Shading signal induces the synthesis of isoflavones in shade-tolerant soybean and accumulates in the leaves of the stressed parts. This study initially revealed the regulation of shading signal (low R / FR) on soybean isoflavones at seedling stage, which laid the foundation for the study on the regulation mechanism of soybean isoflavones in response to shading signals.