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目的 :探讨乙型肝炎病毒慢性感染中C基因启动子 (BCP)变异与慢性重症肝炎的关系。方法 :采用PCR微板核酸杂交结合ELISA检测显示技术 ,检测 74例乙型肝炎病毒慢性感染者BCP区核苷酸 (nt) 176 2碱基A→T和 176 4碱基G→A联合突变。结果 :在 74例乙型肝炎病毒慢性感染者中检出BCP区T176 2A突变 2 4例 (32 .4 % ) ,BCP变异在重症肝炎的发生率为 6 3.6 % (7/ 11)显著高于非重症肝炎的发生率 2 7% (17/ 6 3) (P<0 .0 5 )。结论 :BCP变异似可引起病情加重。
Objective: To investigate the relationship between C gene promoter (BCP) mutation and chronic severe hepatitis in chronic hepatitis B virus infection. Methods: By using PCR microplate nucleic acid hybridization and ELISA, the nucleotide and amino acid sequence of 176 bp and 176 4 bp G → A mutations in BCP region of 74 patients with chronic hepatitis B virus infection were detected. Results: Among 74 chronic hepatitis B patients, 24 (32.4%) T176 2A mutations were detected in BCP area, and the incidence of BCP mutation in severe hepatitis was 6 3.6% (7/11) The incidence of non-severe hepatitis was 27% (17/63) (P <0.05). Conclusion: BCP variation may cause aggravation.