本文介绍酶组织化学中应用冷福尔马林“半固定”方法。此法既可保存细胞内某些酶的活性,叉能使这些酶在细胞内有较好的定位,同一标本可显示组织化学上几种常用的酶,如乳酸脱氢酶、三磷酸腺苷酶、酸性磷酸酶、非特异性酯酶(包括酸性酯酶)、葡萄糖-6-磷酸酶和β-葡萄糖醛酸酶等。 This article describes the use of cold formalin “semi-fixed” method in enzymatic histochemistry. This method can save the activity of some enzymes in the cell, the fork can make these enzymes have a better positioning in the cell, the same specimen can show several commonly used histochemical enzymes, such as lactate dehydrogenase, adenosine triphosphatase, acid Phosphatase, non-specific esterase (including acid esterase), glucose-6-phosphatase and β-glucuronidase.