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酶是参与机体多种化学反应的生物催化剂。在近代生物化学的发展中,酶的应用更加广泛,但酶对热、强酸、强碱、有机溶媒不稳定,很快失活。近年来通过载体活化制备固定化酶,它具有酶特有的催化活性,不溶于水和高的稳定性。因此在研究酶的利用,特别是制作生物化学传染器上是非常有利的。本实验是以光谱纯SiO_2颗粒及含SiO_260%的玻璃纤维膜作为固定脲素酶载体。采用氨基硅烷化和戊二醛交联的方法制备了固定脲素酶的活化载体。经固定化效果检验,固定化
Enzymes are biocatalysts involved in many chemical reactions in the body. In the development of modern biochemistry, the enzyme is more widely used, but the enzyme is unstable to heat, strong acid, strong base and organic solvent, and quickly inactivates. In recent years, immobilized enzymes have been prepared by carrier activation, which have enzyme-specific catalytic activities, are insoluble in water, and have high stability. Therefore, it is very beneficial to study the utilization of enzymes, especially biochemical infectious agents. The experiment is based on pure SiO2 particles and SiO_260% glass fiber membrane as a fixed urease carrier. The active carrier immobilized with urease was prepared by the method of aminosilylation and glutaraldehyde crosslinking. After the test results of immobilization, immobilization