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目的探讨口服低剂量硝酸镧对糖尿病大鼠肝脏PPAR-γ和GLUT4表达的影响。方法以Wistar雄性大鼠为研究对象,将实验动物分为3组即对照组(10只)、糖尿病模型组(18只)和硝酸镧给药组(12只),3组大鼠分别每日给予生理盐水、生理盐水、硝酸镧(0.2 mg/kg)灌胃1个月,然后处死取血清和肝脏。采用ELISA法检测大鼠血清中PPAR-γ和GLUT4的蛋白含量;采用免疫组织化学方法检测大鼠肝脏PPAR-γ和GLUT4的阳性表达强度;采用苏木素-伊红(HE)染色检测肝脏组织的病理改变。结果与对照组比较,硝酸镧给药组和糖尿病模型组,两组大鼠血清中PPAR-γ和GLUT4的蛋白含量及肝脏组织中PPAR-γ和GLUT4的蛋白阳性表达均降低差异有统计学意义(P<0.01);与硝酸镧给药组比较,糖尿病模型组更为显著(P<0.001)。结论口服低剂量硝酸镧(0.2 mg/kg)可通过上调大鼠血清及肝脏PPAR-γ蛋白和GLUT4蛋白,从而对肝组织有一定的保护作用。
Objective To investigate the effects of low dose lanthanum nitrate on the expression of PPAR-γ and GLUT4 in the liver of diabetic rats. Methods Wistar male rats were divided into three groups: control group (n = 10), diabetic model group (n = 18) and lanthanum nitrate group (n = 12) Give saline, saline, lanthanum nitrate (0.2 mg / kg) for 1 month, then sacrifice serum and liver. The protein levels of PPAR-γ and GLUT4 were detected by ELISA. The expression of PPAR-γ and GLUT4 in the liver of rats were detected by immunohistochemical method. The pathological changes of liver tissues were detected by hematoxylin-eosin (HE) staining change. Results Compared with the control group, the protein levels of PPAR-γ and GLUT4 in serum and the protein expression of PPAR-γ and GLUT4 in the liver of the Lanthanum nitrate group and the diabetic model group were significantly lower than those of the control group (P <0.01). Compared with the lanthanum nitrate group, the diabetic model group was more significant (P <0.001). Conclusion Oral administration of low dose of lanthanum nitrate (0.2 mg / kg) can protect liver tissue by up-regulating PPAR-γ protein and GLUT4 protein in serum and liver of rats.