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采用聚乙二醇(PEG)6000模拟干旱的试验方法,从分离自黄淮海地区的19株根瘤菌和2株参比菌株中初步筛选得到能够在该条件下生长良好的根瘤菌7株。进一步进行土壤盆栽干旱胁迫复筛试验,通过根瘤数量、大豆植株干重和含氮量等指标分析,获得了3株耐旱根瘤菌株B.japonicum 4788、B.japonicum 4792和B.japonicum USDA110;测定了在盆栽条件下接种B.japonicum 4792大豆植株耐旱性相关生理的指标,与未接种对照相比,接种根瘤菌的大豆叶片中甜菜碱、SOD酶和叶绿素的含量都有不同程度的增加,丙二醛的含量减少,表明接种根瘤菌可以提高大豆的耐旱性能。
Seventeen Rhizobium strains that could grow well under the conditions were obtained from 19 rhizobial strains and two reference strains isolated from Huang-Huai-Hai region by polyethylene glycol (PEG) 6000 simulated drought. Three soil-resistant rhizobium strains, B.japonicum 4788, B.japonicum 4792 and B.japonicum USDA110, were obtained by further screening experiments of soil potted plants under drought stress. The root nodules, dry weight and nitrogen content of soybean plants were analyzed. Compared with the uninoculated control, the contents of betaine, SOD and chlorophyll in soybean inoculated with Rhizobium were all increased to some extent in the physiological indexes related to drought tolerance of B.japonicum 4792 inoculated with potted plants, Malondialdehyde content decreased, indicating that rhizobium inoculation can improve the drought tolerance of soybean.