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目的检测活动性类风湿性关节炎(RA)患者血清中葡萄糖-6-磷酸异构酶(GPI)含量,研究高水平葡萄糖-6-磷酸异构酶与类风湿性关节炎活动度的关系。方法应用ELISA方法检测89例活动性RA患者、20例骨关节炎患者,20例2-DM患者和30例健康志愿者血清GPI含量,同时检测活动性RA患者血清抗CCP抗体、AKA、RF、CRP、C3和C4。用Spearman’s rho相关分析评价GPI与RA病情活动指标之间的相关性。结果 RA患者组血清中GPI含量为8.33(0.21～4.53)mg/L,明显高于骨关节炎组(0.88±0.68)mg/L、2-DM组0.12(0.05～0.16)mg/L和正常对照组(0.09±0.02)mg/L。GPI含量与DAS-28、RF、ESR、疼痛关节数、关节肿胀疼痛加重时间以及α-胞衬蛋白呈明显正相关关系。结论血清GPI是辅助诊断RA有价值的指标,亦是反映RA病情活动状态的实用指标。
Objective To detect the content of glucose-6-phosphate isomerase (GPI) in the serum of patients with active rheumatoid arthritis (RA) and to study the relationship between high glucose-6-phosphate isomerase and rheumatoid arthritis activity. Methods Serum GPI levels were measured in 89 active RA patients, 20 osteoarthritis patients, 20 patients with 2-DM and 30 healthy volunteers by ELISA. Serum anti-CCP, AKA, RF, CRP, C3 and C4. Spearman’s rho correlation analysis was used to evaluate the correlation between GPI and RA activity. Results The serum GPI level in patients with RA was 8.33 (0.21 ~ 4.53) mg / L, which was significantly higher than that in osteoarthritis patients (0.88 ± 0.68) mg / L and 0.12 (0.05 ~ 0.16) mg / The control group (0.09 ± 0.02) mg / L. There was a positive correlation between GPI content and DAS-28, RF, ESR, painful joint count, painful swelling time of joint swelling, and a-lining protein. Conclusions Serum GPI is a valuable index to assist in the diagnosis of RA and is also a practical indicator of the activity of RA.