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本文用细胞贴附式和内面向外式的膜片箝单通道记录方式研究了小鼠骨髓基质细胞K+通道的动力学特性以及重组人白细胞介素1β(IL1β)对通道的影响,发现了骨髓基质细胞膜上存在一类性质类似于延迟整流的K+通道的电压依赖性K+通道,单通道电导为167±14pS,通道的动力学特性具显著的电压依赖性。1000U/mlIL1β使单通道电导增加到261±36pS,显著增加通道的开放概率,延长开放时间τo2,缩短关闭时间τc2,并诱导通道出现多级开放。研究表明K+通道的激活参与了细胞因子IL1的生物信号转导。
In this study, we investigated the kinetics of K + channels in mouse bone marrow stromal cells and the effect of recombinant human interleukin-1β (IL-1β) on the channels of bone marrow There is a voltage-dependent K + channel on the stromal cell membrane that is similar to the delayed rectifier K + channel with a single-channel conductance of 167 ± 14pS with a significant voltage dependence of the channel kinetics. 1000U / mlIL1β increased the single-channel conductance to 261 ± 36pS, significantly increased the open probability of the channel, prolonged the open time τo2, shorten the close time τc2, and induced multilevel open channels. Studies have shown that K + channel activation is involved in the biological signal transduction of the cytokine IL1.