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采用内皮素建立培养的血管平滑肌细胞增殖模型.用3H胸腺嘧啶核苷(3H－TDR)参入法,流式细胞术,免疫细胞化学及NORTHERNBLOT方法,观察了莲心碱(LIEN)对血管平滑肌细胞增殖的作用及对原癌基因及抑癌基因的影响.结果发现:LIEN能逆转内皮素所致的［3H］TDR参入量增多,阻止血管平滑肌细胞由静止期(G0/G1期)进入DNA合成期(S期)和有丝分裂期((G2/M期),并能逆转内皮素引起的C－FOS,C－MYC,C－SIS原癌基因相关抗原及MRNA表达增强,P53抑癌基因相关抗原及MRNA表达减弱.提示:LIEN能抑制血管平滑肌细胞增殖,与癌基因调控的分子生物学机制有关“,”In order to determine the effects of Liensinine on endothelin (ET)?stimulated proliferation of vascular smooth muscle cells (VSMC) and expression of protooncogene c-myc,c-fos,c-sis and antioncogene P53,the experimental models of proliferation of cultured porcine aortic smooth muscle cells induced by ET were established,and thymidine (TdR) incorporation,flow cytometry,immunohistochemistry and Northern blot assaies were used.The results showed that Liensinine may drop TdR data increased by ET and hold-back V...