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目的:探讨不同强度高压电场对A549肺癌细胞肿瘤转移生物学特性的影响。方法:选择处于生长周期的A549细胞,共分为7个组进行研究,其中A-F组为实验组,G组为不施加电场的空白对照组,A施加500V/cm强度高压电场,F组施加1750V/cm的高压电场,电压场强间隔为250V/cm。采取粘附实验、侵袭及转移实验,检验A549细胞在不同强度高压电场中,其肿瘤生物学转移特性的改变。结果:①各实验组与对照组、各实验组之间的细胞粘附能力,均存在显著性差异(P<0.05);②电场强度≥750V/cm时,各实验组之间、及其与对照组之间的细胞迁移能力,存在显著性差异(P<0.05);③电场强度≥1000V/cm时,各组与对照组间的细胞侵袭能力,存在显著性差异(P<0.05);④电场强度为1000-1250V/cm的各组与1500-1750V/cm各组间的细胞侵袭能力,存在显著性差异,有统计学意义(P<0.05)。结论:不同强度的电场抑制A549肺癌细胞的程度不同,随着强度的增加,A549细胞粘附、迁移和侵袭能力的抑制现象依次出现,并随着电场强度的增加其抑制程度也持续增加。
Objective: To investigate the effects of different electric fields of high intensity on biological characteristics of tumor metastasis in A549 lung cancer cells. Methods: The A549 cells in the growth cycle were selected and divided into seven groups. The AF group was the experimental group, the G group was the blank control group with no electric field applied, the A 500 V / cm intensity high voltage electric field was applied, the F group was 1750V / cm high-voltage electric field, the voltage field strength interval of 250V / cm. Adhesion experiments, invasion and metastasis experiments were performed to test the changes of tumor biological characteristics of A549 cells in different electric fields of high intensity and high intensity. Results ① There was significant difference in cell adhesion between each experimental group and control group and each experimental group (P <0.05); ② When the electric field intensity was ≥750V / cm, There was significant difference (P <0.05) in the ability of cell migration between control groups; ③The cell invasion ability of each group was significantly different from that of control group (P <0.05) when the electric field intensity was above 1000V / cm; ④ There was a significant difference between the groups of electric field intensity of 1000-1250V / cm and the groups of 1500-1750V / cm, which had statistical significance (P <0.05). CONCLUSION: Different intensities of electric field inhibit A549 lung cancer cells to varying degrees. Along with the increase of intensity, the inhibitory effects of adhesion, migration and invasion of A549 cells appear in turn, and the inhibition degree continues to increase with the increase of electric field intensity.