论文部分内容阅读
为了揭示反义硫氧还蛋白基因(anti-trxs)在抗穗发芽小麦中的作用机制;以转反义trxs基因小麦株系为材料,运用生理指标分析的方法,对转基因株系和对照种子萌发过程中硫氧还蛋白h活性、淀粉酶活性、可溶性糖和淀粉含量进行了检测;结果表明,转基因小麦籽粒trxh、α-淀粉酶和β-淀粉酶活性均显著低于对照;淀粉降解和可溶性糖生成速率明显下降。发芽1～5d,转基因小麦种子trxh、α-淀粉酶和β-淀粉酶活性分别比对照下降24.5%、40.4%和23.0%;淀粉降解和可溶性糖生成速率分别比对照降低23.7%和23%;说明,反义trxs基因对转基因小麦籽粒的碳代谢有调控作用。
In order to reveal the mechanism of anti-trxs in spike-resistant wheat, the antisense trxs-transgenic wheat lines were used as materials to analyze the effects of transgenic lines and control seeds The activity of thioredoxin h, the activity of amylase, the content of soluble sugar and starch in the process of germination were tested. The results showed that the activities of trxh, α-amylase and β-amylase in transgenic wheat were significantly lower than those in the control; Soluble sugar production rate decreased significantly. Compared with the control, the trxh, α-amylase and β-amylase activities of transgenic wheat decreased by 24.5%, 40.4% and 23.0%, respectively. The starch degradation and soluble sugar formation rate decreased by 23.7% and 23%, respectively. This indicated that the antisense trxs gene has a regulatory effect on the carbon metabolism of transgenic wheat grains.