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目的:检测原发性高血压(EH)伴颈动脉粥样硬化患者外周血Th17细胞比例的变化及与血管紧张素Ⅱ(AngⅡ)水平的相关性。方法:行颈动脉超声检查选择20例EH伴颈动脉粥样硬化患者(CA组),颈动脉正常的25例EH患者(EH组)以及20例性别、年龄匹配的健康体检者作为对照组。以流式细胞术检测外周血中Th17细胞比例,ELISA法检测血浆中IL-17水平,放射免疫法检测血浆AngⅡ水平。结果:与EH组和对照组相比较,CA组Th17细胞比例显著升高,血浆IL-17水平显著升高。CA组AngⅡ水平高于EH组和对照组,EH组与对照组间AngⅡ水平差异无统计学意义。AngⅡ水平与Th17细胞比例、血浆IL-17水平呈正相关。结论:EH患者的AngⅡ水平和Th17细胞活性升高,这种改变可能与颈动脉粥样硬化的发生发展有关。
Objective: To detect the change of Th17 cell ratio in peripheral blood of patients with essential hypertension (EH) and carotid atherosclerosis and its relationship with the level of angiotensin Ⅱ (Ang Ⅱ). Methods: Twenty patients with EH and carotid atherosclerosis (CA group), 25 normal EH patients (EH group) and 20 healthy and age-matched healthy subjects were selected as the control group. The proportion of Th17 cells in peripheral blood was detected by flow cytometry. The level of IL-17 in plasma was detected by ELISA and the level of plasma AngⅡ by radioimmunoassay. Results: Compared with EH group and control group, the proportion of Th17 cells in CA group was significantly increased and the level of plasma IL-17 was significantly increased. The level of AngⅡ in CA group was higher than that in EH group and control group. There was no significant difference in Ang Ⅱ level between EH group and control group. There was a positive correlation between AngⅡ level and Th17 cell ratio and plasma IL-17 level. Conclusion: The AngⅡ level and the activity of Th17 cells in patients with EH are increased, which may be related to the occurrence and development of carotid atherosclerosis.