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为了首次建立天台鹅耳枥的组织培养与植株再生体系,以其茎段、未萌发的新芽及萌发后的嫩芽、叶片等为外植体进行离体培养试验。结果表明,以新生的嫩芽为外植体,成功诱导不定芽的分化与增殖,最佳培养基配方为1/2 MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+PVP 0.2%。壮苗培养基配方为1/2MS+6-BA 0.5 mg·L-1+NAA 0.05mg·L-1;最佳生根培养基配方为1/4 MS+IBA1.0 mg·L-1+NAA 0.1 mg·L-1。
In order to establish the tissue culture and plant regeneration system of hornbeam stables for the first time, in vitro culture experiments were carried out using stem segments, non-germinating sprouts and post-germination buds and leaves as explants. The results showed that adventitious buds were successfully induced to differentiate and proliferate with new shoots as explants. The best medium was 1/2 MS + 6-BA 2.0 mg · L-1 + NAA 0.2 mg · L-1 + PVP 0.2%. The best rooting medium formula was 1/4 MS + IBA1.0 mg · L-1 + NAA with 1 / 2MS + 6-BA 0.5 mg · L-1 and NAA 0.05 mg · L-1 0.1 mg · L-1.