论文部分内容阅读
已知ATP敏感K~+通道的抑制是胰岛素分泌机制中的重要环节。ATP和或糖代谢产生的其它代谢产物增加,能关闭K~+通道,减少β细胞对K~+的通透性,造成细胞去极化,从而释放胰岛素。作者用K~+通透性指示剂-(86)Rb,研究长时间高糖(16.7mM)处理胰岛后,~(86)Rb的流出量。实验选用雄性Wistar大鼠,分离其胰岛。对照组用5.5mM葡萄糖,高糖组用16.7mM葡萄糖分别培养24小时,然后用0.2mM~(86)Rb温育2小时,洗脱三次后,先用
Known inhibition of ATP-sensitive K ~ + channels is an important part of the insulin secretion mechanism. ATP and / or other metabolites of glucose metabolism increase, can close the K ~ + channel, reduce β cell permeability of K ~ +, resulting in cell depolarization, thus releasing insulin. The author used K ~ + permeability indicator - (86) Rb to study the efflux of ~ (86) Rb after prolonged high glucose (16.7mM) treatment of islets. Male Wistar rats were selected and their islets isolated. The control group was incubated with 5.5 mM glucose and the high glucose group was incubated with 16.7 mM glucose for 24 hours and then incubated with 0.2 mM to 86% Rb for 2 hours. After three washes,