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目的分析2012—2016年珠海市斯坦利沙门菌的耐药情况与脉冲场凝胶电泳(PFGE)分子分型。方法将腹泻病人的粪便进行沙门菌分离培养,生化鉴定和血清学分型,采用药敏纸片法(K-B法)进行12种抗生素的耐药试验。根据美国PulseNet实验室推荐的沙门菌PFGE分型试验方案进行分子分型。结果分离培养共获得斯坦利沙门菌108株,检出高峰期在7—10月,主要感染人群是小于1岁的幼儿(55.6%),感染病例男女比为1.84∶1。药敏试验显示本地斯坦利沙门菌对庆大霉素、三代和四代头孢类抗生素及环丙沙星较为敏感,对复方磺胺类抗生素耐药率为64.8%(70/108),对氨苄青霉素、萘啶酮酸、四环素、氯霉素和链霉素呈现低水平的耐药(10.2%~16.7%),但对链霉素的中度敏感率为68.5%,有18株(16.7%)菌呈多重耐药。108株菌经PFGE分型分为6个聚类簇,共获得66种带型,每种带型包含菌株数1~21株不等,相似值在62.4%~100%,优势带型为ST15(21株)和ST18(10株)。结论珠海市斯坦利沙门菌的耐药情况较为乐观,但对磺胺类抗生素耐药率比较高,也出现多重耐药株,需继续加强监测。斯坦利沙门菌遗传基因多样性,多重耐药株的型别多样,菌株来源广泛。
Objective To analyze the drug resistance of Salmonella Stanis from Zhuhai City in 2012-2016 and the molecular typing of pulsed-field gel electrophoresis (PFGE). Methods The diarrhea patients’ stools were isolated, cultured, biochemically identified and serologically genotyped. Antibiotic resistance tests of 12 antibiotics were performed by K-B method. According to the US PulseNet laboratory recommended Salmonella PFGE typing program for molecular typing. Results A total of 108 strains of Salmonella Stanis were isolated and cultured. The peak period of detection was from July to October. The prevalence was 55.6% in young children less than 1 year old. The ratio of male to female was 1.84:1. Susceptibility tests showed that local Salmonella typhimurium was more sensitive to gentamicin, third and fourth generations of cephalosporins and ciprofloxacin, with a drug resistance rate of 64.8% (70/108) to sulfamethoxazole and ampicillin , Nalidixic acid, tetracycline, chloramphenicol and streptomycin showed a low level of resistance (10.2% ~ 16.7%), but moderate susceptibility to streptomycin 68.5%, 18 strains (16.7%), Multi-drug resistant bacteria. 108 strains were divided into 6 clusters according to PFGE classification, a total of 66 bands were obtained, each strain containing 1 to 21 strains ranging from 62.4% to 100%, the dominant band was ST15 (21 strains) and ST18 (10 strains). Conclusions The drug resistance of Salmonella Stanisulata in Zhuhai is relatively optimistic, but the rate of resistance to sulfa antibiotics is relatively high. Multiple drug-resistant strains also emerge and the surveillance should be strengthened. Stanley Salmonella genetic diversity, multi-resistant strains of various types, strains of a wide range of sources.