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背景:慢性肝病患者常常伴有骨质的丢失,因此探讨预防此种骨丢失对防治此类患者的骨质疏松极有意义。目的:研究甘草酸对肝纤维化小鼠骨丢失的防治作用及机制。设计:以实验动物为研究对象的随机对照研究单位:一所大学的实验动物中心、中心实验室和药理研究室材料:本实验于2001-01/2001-09进行,选择普通级PCR小鼠40只,由本院实验动物中心提供,体质量20～22g,雌雄各半,按质量对等原则随机分为4组,即对照组、模型组、秋水仙组和甘草酸组,每组10只。方法:除对照组外,模型组、秋水仙组和甘草酸组均予400g/L四氯化碳花生油皮下注射5周致小鼠肝纤维化,然后分别应用四氯化碳花生油溶液10mL/kg、秋水仙溶液0.1g/kg和甘草酸溶液0.1mg/kg进行防治,实验结束时摘眼球取血,分离血清检测与肝损伤相关的各种生化指标,观察肝脏病理组织变化以及测定股骨的骨钙(Ca2+)量和其他骨微量元素以及骨羟脯胺酸的含量。主要观察指标:①甘草酸对各组小鼠肝损伤后血清生化指标的影响;②甘草酸对各组小鼠肝脏病理改变的的影响;③甘草酸对各组小鼠骨质的影响;④甘草酸对各组小鼠骨微量元素含量的影响。结果:秋水仙组和甘草酸组小鼠的血清谷草转氨酶(AST)、谷丙转氨酶(ALT)比模型组低(P<0.01);模型组的白蛋白、白蛋白/球蛋白值比秋水仙组和
BACKGROUND: Patients with chronic liver disease are often associated with bone loss. Therefore, it is of great significance to explore prevention of such bone loss in the prevention and treatment of osteoporosis in such patients. Objective: To study the preventive and therapeutic effects of glycyrrhizin on bone loss in mice with liver fibrosis. Design: Randomized controlled trials using experimental animals as research subjects: a university’s laboratory animal center, central laboratory, and pharmacology lab. Materials: This experiment was conducted from 2001-01 to 2001-09, selecting general-grade PCR mice 40 Only, provided by the Experimental Animal Center of our hospital, body weight 20 to 22 g, male and female, were randomly divided into 4 groups according to the principle of quality equivalence, namely control group, model group, colchicum group and glycyrrhizic acid group, 10 in each group. . METHODS: Except for the control group, mice in the model group, colchicine group, and glycyrrhizic acid group were treated with 400 g/L of carbon tetrachloride peanut oil subcutaneously for 5 weeks to induce hepatic fibrosis, followed by the application of carbon tetrachloride peanut oil solution 10 mL/kg, respectively. , Colchicine solution 0.1g/kg and glycyrrhizic acid solution 0.1mg/kg to prevent and control, the end of the experiment eyeballs to take blood, serum separation to detect various biochemical indicators associated with liver injury, observe the pathological changes in the liver and the determination of femoral bone Calcium (Ca2+) content and other bone trace elements and bone hydroxyproline content. MAIN OUTCOME MEASURES: 1 Effect of glycyrrhizic acid on serum biochemical indicators after liver injury in each group; 2 Effect of glycyrrhizic acid on liver pathological changes in mice of each group; 3 Effect of glycyrrhizic acid on bone quality of mice in each group; 4 Effect of glycyrrhizic acid on the content of trace elements in bone in mice. RESULTS: Serum Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were lower in the Colchicum and Glycyrrhizic groups than in the model group (P<0.01). The model group had lower albumin and albumin/globulin values than the colchicum. Group and