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目的观察血塞通预处理对大鼠心肌缺血再灌注(I/R)损伤的早期保护作用,并研究其可能机制。方法采用开胸冠状动脉结扎建立缺血再灌注模型,25只雄性SD大鼠随机分为假手术组,缺血再灌注组(I/R组),缺血预处理组(IPC组),血塞通预处理组(PNS预处理组)。再灌注后2 h测定各组血清肌酸磷酸激酶-MB (CK-MB)含量以及心肌细胞凋亡情况和心肌细胞Bcl-2和Bax蛋白的表达,并观察心肌超微结构变化。结果IPC组及PNS预处理组血清CK-MB均明显低于I/R组(P<0.05)。IPC组及PNS预处理组TUNEL阳性细胞数,Bax阳性细胞数均明显低于I/R组(P<0.05)。和I/R组相比,IPC组及PNS预处理组心肌超微结构损伤减轻。结论PNS预处理后心肌细胞凋亡减少,心肌细胞损伤减轻,对I/R损伤产生有和缺血预处理类似的早期保护作用。
Objective To observe the early protective effect of Xuesaitong preconditioning on myocardial ischemia-reperfusion (I / R) injury in rats and its possible mechanism. Methods Thirty-two male Sprague-Dawley rats were randomly divided into 4 groups: sham operation group, ischemia / reperfusion group (I / R group), ischemic preconditioning group (IPC group), blood Sertone pretreatment group (PNS pretreatment group). At 2 h after reperfusion, the levels of serum creatine phosphokinase-MB (CK-MB), the apoptosis of cardiomyocytes and the expression of Bcl-2 and Bax in myocardial cells were measured and the myocardial ultrastructure was observed. Results Serum CK-MB of IPC group and PNS pretreatment group were significantly lower than that of I / R group (P <0.05). The number of TUNEL positive cells and the number of Bax positive cells in IPC group and PNS pretreatment group were significantly lower than those in I / R group (P <0.05). Compared with I / R group, myocardial ultrastructure damage in IPC group and PNS preconditioning group was alleviated. Conclusions After PNS pretreatment, the apoptosis of cardiomyocytes was reduced and the injury of cardiomyocytes was alleviated. There was a similar early protective effect on ischemic preconditioning against I / R injury.