论文部分内容阅读
１９９５～１９９６年对５个葡萄品种花粉用ＴＴＣ（氯化三苯基四氮唑）法作了如下鉴定研究，（１）葡萄花发育后期４个阶段的花粉生活力；（２）２种干燥条件对花粉生活力的影响；（３）未干燥花粉和干燥花粉不同条件下的保存性。结果表明，（１）葡萄在开花盛期有生活力花粉的比率最高，为５３．１％～８３．５％；（２）温度相同，干燥时间相同，采用８Ｗ日光台灯照射干燥比采用１５Ｗ白炽灯照射干燥有利于保持花粉生活力。干燥后，有生活力花粉的比率分别为１７．２％～３８．５％和３２．２％～５６．５％；比对照降低率分别为５１．８％～７２．６％和２９．３％～４５．２％；（３）未干燥花粉在常温下保存７ｄ和０℃下保存１５ｄ后，有生活力花粉的比率都很低，分别为１．７％～４．５％和２．４％～３．７％，比对照降低率分别为９３．９％～９７．０％和９４．６％～９６．３％。干燥花粉在常温下保存１５ｄ后，有生活力花粉的比率较低，为７．３％～１３．９％，比对照下降为７０．７％～７７．３％，而在０℃下保存３０ｄ后，有生活力花粉的比率仍较高，为２６．８％～３９．４％，比对照下降率为５．６％～３０．３０％。
Five grapevine pollens were identified by TTC (triphenyltetrazolium chloride) from 1995 to 1996 as follows: (1) Pollen viability in four stages of grapevine development; (2) Two kinds of dry Conditions on the pollen viability; (3) Unserved and dried pollen under different conditions of preservation. The results showed that: (1) The percentage of vitality of pollen in flowering stage was the highest (53.1% -83.5%); (2) The same temperature and same drying time, Light irradiation drying is conducive to maintaining pollen viability. After drying, the viable pollen rates were 17.2% -38.5% and 32.2% -56.5%, respectively, and the rates of reduction were 51.8% -72.6% and 29.3% respectively % ~ 45.2%. (3) The percentage of viable pollen of the non-dried pollen stored at room temperature for 7 days and at 0 ℃ for 15 days were 1.7% -4.5% and 2%, respectively. 4% ~ 3.7%, the reduction rates were 93.9% ~ 97.0% and 94.6% ~ 96.3% respectively. The percentage of viable pollen was 7.3% ~ 13.9% when the dry pollen was stored at room temperature for 15 days, which was 70.7% ~ 77.3% lower than that of the control and kept at 0 ℃ for 30 days The viability of pollen is still high at 26.8% -39.4%, which is 5.6% -30.30% lower than the control.