论文部分内容阅读
目的:通过细胞摄取巴氯酚的体外药动学研究及液质联用定量检测方法的建立,为确定巴氯酚更合理的给药途径和跨越血脑屏障的机制研究提供依据。方法:利用CHO-K1细胞摄取药物的细胞生物学实验方法结合液质联用检测技术,以细胞/介质(cell/medium,C/M)比值为指标进行CHO-K1细胞摄取巴氯酚体外药动学研究,并进行方法学考察。本实验选取的摄取时间点分别为30s,1,5,30,60min。结果:液质联用定量方法灵敏度高,重复性好。在0～30min区间内,细胞摄取巴氯酚的量随时间几乎呈线性增加,30min时的C/M值达到最高为9.74(n=4),基本达到饱和状态,30～60min区间,细胞摄取巴氯酚的量几乎不再随时间发生变化。结论:为深入研究巴氯酚跨越血脑屏障的机制并确定实际应用的给药途径提供数据基础和参考依据。定量方法简便快捷,灵敏度高,重复性好,可广泛应用于细胞摄取微量药物的检测研究中,具实用价值。
OBJECTIVE: To establish a pharmacokinetic study of cytochrome catabolism in vitro and to establish a quantitative detection method for LC-MS. Methods: The cytotoxicity of CHO-K1 cells in vitro was determined by cytochemical assay of CHO-K1 cells combined with MS / MS and the cell / medium ratio (C / M) Kinematics research, and methodological study. The time points selected for this experiment were 30s, 1,5,30,60 min. Results: LC / MS method with high sensitivity and good reproducibility. During the period of 0-30 minutes, the amount of cellophane uptake by cells increased almost linearly with time, reaching a maximum value of 9.74 (n = 4) at 30 minutes, reached the saturation level basically. At 30-60 minutes, cell uptake The amount of bachol hardly changed over time. Conclusion: This study provides a data basis and a reference basis for further study of the mechanism of BPH crossing the blood-brain barrier and to determine the practical route of administration. Quantitative method is simple, rapid, high sensitivity, reproducibility, can be widely used in the detection of trace drug uptake of cells, with practical value.