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目的 探讨临床相关浓度异丙酚对培养人血管内皮细胞及其受联胺诱导脂质过氧化损伤时的保护作用。方法 将培养人脐静脉内皮细胞３～４ 代于融合状态，分为不加联胺正常培养及加联胺损伤两组，分别向两组中加入０、１２．５、２５、５０及１００μｍ ｏｌ／Ｌ异丙酚作用３０分钟，再向联胺损伤组中加入１．０×１０－ ４ｍ ｏｌ／Ｌ联胺作用８０ 分钟。测定各组丙二醛（ＭＤＡ）浓度，分别进行光镜及扫描电镜观察。结果 随着异丙酚浓度的升高，受联胺损伤内皮细胞的ＭＤＡ 含量明显下降，内皮细胞的损伤程度明显减轻。高浓度异丙酚明显降低基础ＭＤＡ 的产生。结论 临床相关浓度的异丙酚对内皮细胞过氧化损伤有明显的保护作用。
Objective To investigate the protective effects of propofol at clinically relevant concentrations on cultured human vascular endothelial cells and their effects on lipid peroxidation induced by hydrazine. Methods Human umbilical vein endothelial cells (HEKs) were cultured for 3-4 generations in the fusion state, and were divided into two groups: normal control group without adding amines and amine-induced injury. Groups of 0,12.5,25,50 and 100μmol / L propofol for 30 minutes, and then to the group of amine damage added 1.0 × 10-4mol / L hydrazine for 80 minutes. The concentration of malondialdehyde (MDA) in each group was measured and observed by light microscope and scanning electron microscope respectively. Results With the increase of propofol concentration, the MDA content of endothelial cells injured by hydrazine decreased significantly, and the damage of endothelial cells was significantly reduced. High concentrations of propofol significantly reduced basal MDA production. Conclusion Propofol at a clinically relevant concentration has a significant protective effect on endothelial cell peroxidation injury.