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目的 探讨血管紧张素转换酶 (ACE)基因插入缺失多态性对肺原性心脏病 (简称肺心病 )易感性、血管重构和肺功能损害程度的影响。方法 从外周血白细胞中提取人类基因组DNA ,应用ACE基因第 16内含子多态区两侧序列作为引物 ,采用PCR方法测定 6 2例肺心病患者、6 0例慢性阻塞性肺病 (COPD)患者、12 6例健康者的ACE基因型。用多元线性回归分析纠正性别、年龄等影响因素后确定独立危险因子。结果 肺心病组、COPD组中DD基因型分布频率为 0 .38、0 .4 3,与正常对照组的 0 .2 7比较具有显著性差异 (P <0 .0 1) ;也具有更高的D等位基因频率 0 .6 1、0 .6 2 ,与正常对照组 0 .4 4比较有显著差异性 (P <0 .0 1) ;DD型肺心病患者肺通气功能损害较Ⅱ型者为重 (P <0 .0 5 )。结论 ACE基因D型纯合子可能与肺心病的遗传易感性相关 ,DD型基因可能是肺心病、COPD发病新的独立危险因素
Objective To investigate the effect of angiotensin converting enzyme (ACE) gene insertion deletion polymorphism on the susceptibility to pulmonary heart disease (pulmonary heart disease), vascular remodeling and pulmonary function impairment. Methods Human genomic DNA was extracted from peripheral blood leukocytes. PCR was used to detect 62 cases of patients with cor pulmonale and 60 cases of patients with chronic obstructive pulmonary disease (COPD) by using the 16th intron polymorphism region of ACE gene sequence. , 12 6 healthy subjects ACE genotype. Multivariate linear regression analysis to correct gender, age and other influencing factors to determine the independent risk factors. Results The frequencies of DD genotypes in COPD group and COPD group were 0.38,0.43, which were significantly different from those of the normal control group (P <0. 01), and also higher The frequency of D allele was 0.6160.626, which was significantly different from that of the normal control group (P <0.01). The pulmonary dysfunction in patients with DD-type pulmonary heart disease was more severe than that in type-Ⅱ (P <0.05). Conclusion D-homozygote of ACE gene may be associated with genetic susceptibility to pulmonary heart disease. DD-type gene may be a new independent risk factor for pulmonary heart disease and COPD