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目的:建立广金钱草及其制剂的高效液相色谱(HPLC)指纹图谱,为其质量评价提供依据。方法:色谱柱为迪马ODS-C18(250mm×4.6mm,5μm),流动相为甲醇-2%磷酸溶液(梯度洗脱),流速为1.0mL·min-1,柱温为25℃,检测波长为360nm,记录广金钱草及其制剂的HPLC指纹图谱。结果:广金钱草药材和制剂均有13个共有峰,多数峰可以达到较好分离。结论:本方法具有良好的精密度、稳定性和重复性,建立的指纹图谱检测标准可以作为广金钱草及其制剂质量评价的主要依据之一。
Objective: To establish HPLC fingerprinting of Desmodium gravetifolia and its preparations to provide the basis for its quality evaluation. Methods: The column was Dima ODS-C18 (250mm × 4.6mm, 5μm). The mobile phase consisted of methanol-2% phosphoric acid solution (gradient elution) at a flow rate of 1.0mL · min- Wavelength of 360nm, record HPLC fingerprinting Schefflera major and its preparation. Results: There were 13 common peaks and the majority of peaks could be separated well. Conclusion: The method has good precision, stability and repeatability. The established fingerprint detection standard can be used as one of the main bases for the quality evaluation of.