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目的观察硝酸甘油诱导的偏头痛大鼠模型不同时间点过氧化物酶体增殖物受体(PPARs)在三叉神经颈复合体(TCC)中的表达情况。方法按随机数字表法将SD大鼠分为模型组(n=12,腹部皮下注射硝酸甘油10 mg·kg~(-1),造模)和对照组(n=12,腹部皮下注射等量生理盐水)。两组按时间点分别分为2h、4h模型亚组和2h、4h对照亚组。造模后2h和4h两组各取6只大鼠,免疫组化法观察TCC内PPAR各亚型的表达。结果造模后,2h模型组与对照组比较PPARα表达减少,4h模型组与对照组和2h模型组比较PPARα表达增加,随时间延长表现为先降低后升高的趋势。2h模型组和4h模型组与对照组比较PPARβ/δ表达均增加,2h模型组与4h模型组比较PPARβ/δ表达差异无显著性。2h模型组与2h对照组比较PPARγ表达明显增多,4h模型组与2h对照组比较PPARγ表达水平差异无显著性。结论 PPARs各亚型在偏头痛大鼠模型发病中占有重要地位,不同时间节点表达情况不尽相同。
Objective To observe the expression of peroxisome proliferator - activated receptors (PPARs) in trigeminal neuralgia complex (TCC) at different time points in nitroglycerin - induced migraine rat model. Methods SD rats were randomly divided into model group (n = 12, subcutaneous nitroglycerin 10 mg · kg -1, model) and control group (n = 12, Saline). The two groups were divided into 2h, 4h model subgroups and 2h, 4h control subgroups according to the time points. Six rats in each group were taken at 2h and 4h after modeling, and the expression of PPAR subtypes in TCC were observed by immunohistochemistry. Results After modeling, the expression of PPARα in model group was lower than that in control group at 2h. Compared with control group and model group at 2h, the expression of PPARα increased at 4h and decreased at first and then increased with time. The expression of PPARβ / δ in 2h model group and 4h model group increased compared with that in control group, and there was no significant difference in PPARβ / δ expression between 2h model group and 4h model group. 2h model group compared with 2h control group PPARγ expression increased significantly, 4h model group compared with 2h control group PPARγ expression level was no significant difference. Conclusion The subtypes of PPARs play an important role in the pathogenesis of migraine rat model. The expression of PPARs in different time points is different.