论文部分内容阅读
目的:探讨转化生长因子-β1(TGF-β1)分子表达水平及其编码区基因多态性与多发性骨髓瘤(Mu ltip le myelo-m a,MM)的关系。方法:应用ELISA方法检测标本血浆TGF-β1的浓度,应用L ightCyc ler实时荧光PCR结合熔点曲线分析的方法检测转化生长因子-β1编码区3个基因多态性:codon10 T>C(Leu10Pro)、codon25 G>C(Arg25Pro)和codon263 C>T(Thr263 Ile)。结果:患者血浆TGF-β1浓度明显高于对照组,但TGF-β1基因codon10位点各种基因型和等位基因在对照组和MM患者组以及MM患者各型之间的分布频率并没有显著差异,而且MM患者各种基因型血浆TGF-β1的浓度也没有显著差异,TGF-β1基因codon25和codon263位点均不存在预想中的基因多态性。结论:转化生长因子-β1可能在多发性骨髓瘤的发生中起到一定的作用,但该因子基因编码区多态性并不会影响血浆TGF-β1的浓度,也与MM的发生以及亚型没有明确的相关性。
Objective: To investigate the relationship between the expression of transforming growth factor-β1 (TGF-β1) gene and its coding region gene polymorphisms and multiple myeloma (MM). Methods: The plasma concentration of TGF-β1 was detected by ELISA. The polymorphism of TGF-β1 in the coding region of three genes was detected by LightCyc ler real-time PCR and melting curve analysis: codon10 T> C (Leu10Pro) codon25 G> C (Arg25Pro) and codon263 C> T (Thr263 Ile). Results: The concentration of TGF-β1 in plasma was significantly higher than that in control group. However, there was no significant difference in the frequency of genotypes and alleles of codon10 in TGF-β1 between the control group and MM patients and the MM patients There was no significant difference in the plasma concentrations of TGF-β1 between the various genotypes of MM patients. There were no expected polymorphisms in the codon25 and codon263 loci of TGF-β1. CONCLUSION: Transforming growth factor-β1 may play a role in the pathogenesis of multiple myeloma. However, the polymorphism of coding region of this factor does not affect the concentration of plasma TGF-β1, but also with the occurrence of MM and its subtype No clear relevance.