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目的探讨遗人类白细胞抗原(human leukocyte antigen,HLA)DQ基因多态性与丙型肝炎感染转归的关联。方法应用Taq Man探针方法检测339例丙型肝炎病毒(hepatitis C virus,HCV)持续感染者,262例HCV自限感染者和942例健康对照者HLA-DQ基因rs2856718和rs7453920位点的基因多态性,比较不同基因型与丙型肝炎病毒感染转归的关系。结果采用多因素Logistic回归分析,结果显示rs2856718位点杂合基因型AG在感染组中的频率均低于对照组(调整OR=0.73,95%CI:0.56~0.94);另外,rs7453920 AA基因型可增加个体对HCV的易感性(调整OR=1.75,95%CI:1.01~3.03)。结果显示,与AG单倍型相比,携带AA单倍型的个体可增加丙型肝炎易感性(调整OR=1.67,95%CI:1.11~2.52)。分层分析结果发现,在男性人群中,rs2856718位点的杂合基因型AG在感染组中的频率低于对照组(调整OR=0.68,95%CI:0.51~0.93);在45~60岁人群中,rs7453920位点突变纯合基因型GG在感染组中的频率高于对照组(调整OR=2.35,95%CI:1.11~4.98)。结论 HLA-DQ基因多态性与丙型肝炎病毒感染转归存在关联。
Objective To investigate the association of genetic polymorphisms of human leukocyte antigen (HLA) DQ with the prognosis of hepatitis C infection. Methods A total of 339 HLA-DQ gene rs2856718 and rs7453920 loci were detected in 339 cases of persistent HCV infection, 262 cases of HCV self-limited infection and 942 healthy controls by TaqMan probe. The relationship between different genotypes and the outcome of hepatitis C virus infection was compared. Results The multivariate logistic regression analysis showed that the frequencies of rs2856718 heterozygous genotype AG in the infected group were lower than those in the control group (adjusted OR = 0.73, 95% CI: 0.56-0.94). In addition, rs7453920 AA genotype Can increase individual susceptibility to HCV (adjusted OR = 1.75, 95% CI: 1.01 ~ 3.03). The results showed that individuals carrying the AA haplotype had an increased susceptibility to hepatitis C compared with the AG haplotype (adjusted OR = 1.67, 95% CI: 1.11-2.52). In stratified analysis, the frequencies of rs2856718 heterozygous genotype AG in the infected group were lower than those in the control group (adjusted OR = 0.68, 95% CI: 0.51-0.93) in the male population; at the age of 45-60 In the population, the frequency of homozygous genotype G at rs7453920 mutation in the infected group was higher than that in the control group (adjusted OR = 2.35, 95% CI: 1.11-4.98). Conclusion HLA-DQ gene polymorphism is associated with the prognosis of hepatitis C virus infection.