以Bcl-2为靶标siRNA提高HL-60细胞对三氧化二砷敏感性的探讨(英文)

来源 :Chinese-German Journal of Clinical Oncology | 被引量 : 0次 | 上传用户:qqtigert123
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Objective: To study whether the small-interference RNA (siRNA) targeting against Bcl-2 gene can enhance sensi-tivity of HL-60 cell to arsenic trioxide. Methods: SiRNA was transferred into the HL-60 cells. At 6 h after transfection, the cells were cultured with arsenic trioxide. The cell growth of the HL-60 cells was detected using MTT at 24, 48 and 72 h, respectively. The levels of the Bcl-2 protein and reactive oxygen species (ROS), as well as of the membrane potential of the mitochondrion were determined by flow cytometry. Results: The Bcl-2 siRNA significantly increased the inhibitory action of arsenic trioxide on growth of HL-60 cells. The combination of siRNA with arsenic trioxide resulted in decrease of the Bcl-2 protein level and increase of the ROS level, as well as significant descending of the membrane potential of mitochondrion of HL-60 (P < 0.05). Conclusion: The siRNA targeting Bcl-2 can increase the sensitivity of the HL-60 leukemia cells to arsenic trioxide by inhibiting the expression of Bcl-2 protein. Objective: To study whether the small-interference RNA (siRNA) targeting against Bcl-2 gene can enhance sensi-tivity of HL-60 cell to arsenic trioxide. Methods: SiRNA was transferred into HL-60 cells. At 6 h after transfection The cells were cultured with arsenic trioxide. The cell growth of the HL-60 cells was detected using MTT at 24, 48 and 72 h, respectively. The levels of the Bcl-2 protein and reactive oxygen species (ROS), as well as of the membrane potential of the mitochondria were determined by flow cytometry. Results: The Bcl-2 siRNA significantly increased the inhibitory action of arsenic trioxide on growth of HL-60 cells. The combination of siRNA with arsenic trioxide resulted in a decrease of the Bcl -2 protein level and increase of the ROS level, as well as significant descending of the membrane potential of mitochondrion of HL-60 (P <0.05). Conclusion: The siRNA targeting Bcl-2 can increase the sensitivity of the HL-60 leukemia cells to arsenic trioxide by inhibiting the expression of Bcl-2 protein.
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