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从患病美蛙的肝组织中分离得到一个菌株,命名为蛙-990529。用该菌株对健康蛙进行攻毒,该健康蛙表现出与自然发病的症状相同的病症,且有较强的致死毒性,表明蛙-990529为病原菌。该菌种在普通营养琼脂上生长较慢,25℃培养24h后,菌落直径为0.5mm,圆形,隆起,湿润,半透明,呈露滴状。肉汤培养基中呈粒状生长。半固体琼脂中生长线呈直线。氧化酶阴性、OF不发酵、无动力;采用API-20NE试条鉴定为洛菲氏不动杆菌。该菌对四环素、强力霉素、新霉素等药物敏感。筛选研制的“蛙血康”药物,在临床应用中显示出良好的防治效果。
A strain was isolated from the liver tissue of a diseased frog and named frog -990529. The healthy frogs were challenged with this strain. The healthy frog showed the same symptoms as the naturally occurring ones and had a strong lethal toxicity, indicating that frog-990529 is a pathogen. The strain grows slowly on common nutrient agar. After culturing at 25 ° C for 24 hours, the colony has a diameter of 0.5 mm, round, raised, moist, translucent and dew drops. Bouillon medium was granular growth. Semi-solid agar growth line was linear. Oxidase negative, OF not fermented, no motivation; API-20NE test strip identified as Loh acuminate. The bacteria tetracycline, doxycycline, neomycin and other drugs sensitive. Screening of “frog blood Kang” drugs, in clinical applications showed good control effect.