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目的 :建立测定赤土茯苓中赤土茯苓苷含量的方法。方法 :荧光分光光度法。用无水乙醇作溶媒 ,以Ex =388nm ,Em =5 0 3nm测定荧光强度。结果 :浓度在 0 .4～ 8.0 μg/ml范围内 ,浓度与荧光强度呈良好线性关系 ,回归方程为F =0 .316 1+ 6 .86 15C ,r =0 .9995。平均加样回收率为 99.14% ,RSD为 0 .98%。测得赤土茯苓中赤土茯苓苷的含量为 0 .5 3 %。结论 :本法快速、简便、灵敏 ,可用于赤土茯苓药材及制剂的含量测定及质量控制
Objective : To establish a method for determining the content of terraeoside in terracotta. Methods: Fluorescence spectrophotometry. Using absolute ethanol as a solvent, the fluorescence intensity was measured at Ex = 388 nm and Em = 503 nm. Results: The concentration was in the range of 0.4～8.0 μg/ml. The concentration and fluorescence intensity showed a good linear relationship. The regression equation was F=0.316 1+ 6 .86 15C, r=0.9995. The average recovery rate was 99.14% with an RSD of 0.98%. The content of terraeoberin in terra cotta was 0.53%. Conclusion : This method is rapid, simple and sensitive. It can be used for the determination and quality control of terracotta medicine and its preparations.