采用RAPD PCR技术分析了 4个绿僵菌菌株的DNA遗传多态性。从 1 60条引物中筛选出 2 7条引物 ,对各菌株进行PCR扩增 ,结果 4个绿僵菌菌株共扩增出 334个位点 ,其中多态性位点 2 99个 ,占 89 5 % ,表明各菌株间具有较丰富的遗传多态性。菌株DNA多态性与原寄主和孢子形态表现出明显的相关性 ,但与对松墨天牛幼虫的毒力间未表现出相关性。图2表 4参 7 The DNA genetic polymorphisms of four strains of Metarhizium anisopliae were analyzed by RAPD PCR. Twenty-seven primers were screened from 160 primers, and PCR amplification was performed on each strain. As a result, 334 strains of four Metarhizium strains were amplified, of which 2 99 were polymorphic, accounting for 89 5 %, Indicating that each strain has a rich genetic polymorphism. The DNA polymorphism of the isolates showed obvious correlation with the original host and spore morphology, but not with the virulence of Monochamus alternatus larvae. Figure 2 Table 4 Reference 7