人肝肿瘤细胞株 Hep G2可应用于体外遗传毒理学试验等多种研究。但其培养难度较大 ,通过对 Hep G2细胞在不同培养条件下生长状态的对比 ,探讨了 Hep G2细胞培养增殖的最佳条件。结果表明在下列情况下细胞能顺利传代生长 :复苏时需 2 0 %胎牛血清 ,接种密度为 1× 10 4/ cm2 ,需在含 5 % CO2 环境下 ;培养时需 10 %胎牛血清 ,可在无 5 % CO2环境下 Human hepatoma cell line Hep G2 can be used in a variety of studies such as in vitro genotoxicity assays. However, it is difficult to culture Hep G2 cells in Hep G2 cells under different culture conditions by comparing the growth of Hep G2 cells cultured to explore the best conditions. The results showed that the cells could successfully pass the subculture under the following conditions: 20% fetal bovine serum was required for resuscitation, the inoculation density was 1 × 10 4 / cm 2, and the culture medium required 10% fetal bovine serum, Available in 5% CO2-free environment