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本工作给清醒、自由活动的SD雄性大鼠第三脑室内注射血管紧张素Ⅱ,用放射免疫测定给药前后血浆催乳素(PRL)和β-内啡肽(β-EP)含量的变化,结果显示,注射ANGⅡ50和500ng,可显著升高血浆PRL和β-EP的含量。静脉注射多巴胺受体阻断剂spiroperidol后,也可显著升高血浆PRL水平,若在此基础上加脑室内注射50ng ANGⅡ,血浆PRL含量与单独静脉注射spiroperidol无显著差别。在体外,ANGⅡ作用于前叶垂体细胞后,可使其培养液中PRL和β-EP含量显著升高。用EGTA络合细胞外Ca~(2+)后,不影响10~(-8)M ANGⅡ所致的PRL升高,但部分抑制其升高β-EP的效应。10~(-8)M的ANGⅡ可使垂体细胞内Ca~(2+)浓度显著升高,但不影响其胞内cAMP的含量。
In this study, the third intraventricular injection of angiotensin II into awake, free-moving SD male rats was performed by radioimmunoassay before and after treatment. Changes of plasma prolactin (PRL) and β-endorphin (β-EP) The results showed that injection of ANG Ⅱ50 and 500ng, can significantly increase plasma levels of PRL and β-EP. Intravenous injection of dopamine receptor antagonist spiroperidol, but also significantly increased plasma PRL levels, if on this basis plus intraventricular injection of 50ng ANG Ⅱ, plasma PRL content and intravenous spiroperidol no significant difference. In vitro, ANG Ⅱ effect on anterior pituitary cells, can make its culture medium PRL and β-EP content was significantly increased. After EGCTA complexed with extracellular Ca ~ (2+), it did not affect the increase of PRL induced by 10 ~ (-8) M ANGII, but partially inhibited its effect of increasing β-EP. Ang Ⅱ at 10 ~ (-8) M significantly increased intracellular Ca ~ (2+) concentration in pituitary cells but did not affect intracellular cAMP levels.