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人类体液免疫可对抗龋病。目前葡糖基转移酶(glucosyltransferases,GTFs)是研究得最为广泛的抗原物质。一些研究表明龋病状态与抗GTFs抗体水平或抗体对GTFs酶活动的抑制程度之间具有正相关关系。抗体介导的免疫反应可能通过与细菌表面抗原反应抑制聚集或通过抑制GTFs所催化的葡聚糖合成来控制致龋菌。本文旨在获得人类对S.mutans抗体反应的全貌及寻找中国人群中与无龋有关的特异抗体反应。 材料和方法 随机选取18～20岁中国台湾大学学生为研究对象,据患龋情况和唾液中S.mutans数量分为龋活跃组(77人)和无龋组(80人)。从有血清c型变形链球菌感染的个体收集唾液和血标本共108例,分别离心后冷冻保存。用脑心肉汤培养S.mutans MT-8148株,提取制备胞外蛋白质抗原(EXP-A)和胞壁相关性蛋白质抗原(CWP-A),SDS-PAGE法分离其蛋白质。
Human humoral immunity can fight against dental caries. At present, glucosyltransferases (GTFs) are the most widely studied antigens. Some studies have shown a positive correlation between cariogenic status and anti-GTFs antibody levels or the extent to which antibodies inhibit the activity of GTFs. Antibody-mediated immune responses may control cariogenic bacteria by inhibiting aggregation by reacting with bacterial surface antigens or by inhibiting dextran synthesis catalyzed by GTFs. The purpose of this article is to obtain a complete picture of the human response to S. mutans and to find the specific antibody response to caries-free in the Chinese population. MATERIALS AND METHODS Randomly selected students aged 18-20 years old from Taiwan University of China as the study subjects. According to the status of caries and the number of S.mutans in saliva, they were divided into caries-active group (77 persons) and caries-free group (80 persons). A total of 108 cases of saliva and blood samples were collected from individuals infected with serotype C Streptococcus mutans and were respectively centrifuged and cryopreserved. S.mutans MT-8148 strain was cultured with brain-heart bouillon, extracellular protein antigen (EXP-A) and cell wall-associated protein antigen (CWP-A) were extracted and the proteins were separated by SDS-PAGE.