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新型隐球菌是广泛存在于外界的有荚膜真菌,对人类致病,包括从无症状肺部感染到致死性播散性疾病,后者主要见于免疫性显著降低的患者。鉴于本菌分布广,但极少人得病,提示人体对之有强大的正常防御。在小鼠模型中,过去已证实巨噬细胞和多核粒细胞有杀伤作用。近年来Murphy等又提出NK细胞是第三种防御细胞,能在体外抑制此菌的生长,但抑制方法不明。本文目的是用体外法探讨这一机理。作者先用Murphy等法,从7～8周龄CBA小鼠脾细胞分出尼龙毛不粘附细胞,用Percoll不连
Cryptococcus neoformans is a capsular fungus that is widespread outside and causes disease in humans, ranging from asymptomatic pulmonary infections to lethal disseminated diseases, the latter seen mainly in patients with markedly reduced immunity. In view of the wide distribution of the bacteria, but very few people get sick, suggesting that the body has a strong normal defense. In mouse models, macrophages and multinucleated granulocytes have been shown to be cytotoxic in the past. In recent years, Murphy et al. Have also suggested that NK cells are the third type of defense cells that can inhibit the growth of this bacterium in vitro, but the inhibition method is unknown. The purpose of this paper is to explore this mechanism in vitro. The authors used Murphy et al. To separate nylon hair nonadherent cells from 7-8 week-old CBA mouse splenocytes,