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[Objective] To detect the optimal extraction technology of total saponins from tomato by pectinase method. [Method] With the concentration of saponins A as the index, single factor test was conducted with the four factors of reaction temperature, reaction time, pH value and enzyme amount. Based on this, L9(34) orthogonal test was designed. [Result] The optimal extraction condition was 55 ℃ extraction temperature, 2 h reaction time, 0.4‰ enzyme amount and pH 4.0. Under these conditions, concentration of saponins A was the maximum. Verification test showed that under the selected conditions, the average yield of total saponins in enzyme group was 0.25%, which was significantly lower than that in no enzyme group. [Conclusion] This was the optimal technology to extract saponins from tomato, and could effectively enhance the yield of tomato saponins and reduce the viscosity of extracting solution.
[Objective] To detect the optimal extraction technology of total saponins from tomato by pectinase method. [Method] With the concentration of saponins A as the index, single factor test was conducted with the four factors of reaction temperature, reaction time, pH value and Based on this, L9 (34) orthogonal test was designed. [Result] The optimal extraction condition was 55 ° C extraction temperature, 2 h reaction time, 0.4% enzyme amount and pH 4.0. Under these conditions, the concentration of saponins A was the maximum. Verification test showed that under the selected conditions, the average yield of total saponins in enzyme group was 0.25%, which was significantly lower than that in no enzyme group. [Conclusion] This was the optimal technology to extract saponins from tomato , and could effectively enhance the yield of tomato saponins and reduce the viscosity of extracting solution.