小豆辐射诱变效应的研究Ⅲ~(60)Co-r射线不同剂量处理小豆种子的细胞遗传学效应

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本试验用60Co-r射线吸收剂量0~700Gy处理小豆风干种子,对其M1代根尖细胞染色体畸变类型及畸变率、M1代植株花粉育性和主要农艺性状进行观察,结果表明:M1代根尖细胞异常率达10%、植株花粉育性达50%时的吸收剂量为400Gy左右。),除多极外,其余2种畸变类型的频率与剂量间的关系也达极显著,并可用直线回归方程加以描述:桥:Y=0.89+0.0129x(r=0.9096):断片:Y=1.16+0.0056x(r=0.9584);总的畸变:Y=1.36+0.020x(r=0.941),如图1。野生小豆染色体极小,未能观察。2.260Co-r射线处理对小豆M1、M2、M3代植株花粉育性的影响在小豆开花期取植株外形生长较为正常的植株上的花粉进行镜检,结果表明:栽培小豆的花粉育性随辐射吸收剂量的加大而育性降低很快,呈显著的直线回归关系(图2),而野生小豆未进行辐射处理的植株花粉其本身的育性就相当低,仅为32.5%,进行辐射处理后其花粉育性略有下降,400Gv以内仅比对照下降30%以下,处理剂量达到500Gy,花粉育性大幅度下降,为对照的34%,其花粉育性仅为11.0%,处理剂量达700Gy的植株上开花极少,几? In this experiment, dried seeds of adzuki bean were treated with 60Co-r ray absorbed dose of 0 ~ 700Gy, the type of chromosome aberration and the rate of aberration of M1 root tip cells, the pollen fertility of M1 plants and the main agronomic characters were observed. The results showed that: Abnormal rate of tip cells up to 10%, plant pollen fertility up to 50% of the absorbed dose of about 400Gy. ). Except for multipole, the relationship between the frequency and dose of the other two types of distortion was also significant, and can be described by the linear regression equation: Bridge: Y = 0.89 + 0.0129x (r = 0.9096) : Y = 1.16 + 0.0056x (r = 0.9584); Total distortion: Y = 1.36 + 0.020x (r = 0.941) The wild adzuki bean chromosome is very small, failed to observe. 2.260Co-r ray treatment of adzuki bean M1, M2, M3 generation plant pollen fertility in the adzuki bean flowering stage to take shape more normal plant pollen microscopy, the results show that: Adzuki bean pollen fertility with Radiation absorbed dose increased fertility decreased rapidly, showing a significant linear regression (Figure 2), while wild adzuki bean radiation treatment plant pollen itself is very low fertility, only 32.5% The pollen fertility declined slightly after treatment with 400Gv, but the treatment dose reached 500Gy, the pollen fertility dropped significantly to 34% of the control, the pollen fertility was only 11.0% , The flowering of plants on the treatment dose of 700Gy very few, how many?
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