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目的:研究黄连总碱(TAL)对小鼠结肠炎的作用及其机制。方法:实验设正常、模型、柳氮磺胺吡啶组(SASP,520mg/kg)和TAL组(150mg/kg)。正常组小鼠饮用蒸馏水,其余组自由饮用4%葡聚糖硫酸钠(DSS)水溶液,同时分别灌胃给予溶剂或干预药物(0.2ml/10gwt,1次/d×7d)。记录小鼠疾病活动指数(DAI);测定结肠组织MDA含量,SOD、MPO活性及ICAM鄄1、NF鄄κBp65表达水平。结果:模型组DAI显著增高,结肠粘膜损伤严重;MDA含量、MPO活性及ICAM鄄1和NF鄄κBp65表达明显升高,SOD活性下降(P<0.01)。SASP520mg/kg能明显逆转上述改变;TAL150mg/kg具有相似的作用。结论:TAL可能通过抗氧自由基作用,抑制炎性细胞活化、迁移及NF鄄κB激活,缓解小鼠结肠炎症。
Objective: To study the effect of total alkaloids from berberine (TAL) on colitis in mice and its mechanism. METHODS: Normal, model, sulfasalazine (SASP, 520 mg/kg) and TAL (150 mg/kg) were used in the experiment. The normal group of mice drink distilled water, the remaining group free drinking 4% dextran sodium sulfate (DSS) aqueous solution, while intragastric administration of solvent or intervention drug (0.2ml/10gwt, 1 / d × 7d). The mouse disease activity index (DAI) was recorded; the content of MDA in the colon tissue, SOD, MPO activity, and the expression levels of ICAM1 and NF-κBp65 were measured. RESULTS: The DAI in the model group was significantly increased, and the mucosal damage in the colon was severe; the MDA content, MPO activity, and the expression of ICAM1 and NF-κBp65 were significantly increased, and the SOD activity was decreased (P<0.01). SASP 520mg/kg can significantly reverse the above changes; TAL150mg/kg has a similar effect. Conclusion: TAL may relieve the colonic inflammation of mice by inhibiting the activation of inflammatory cells and the activation of NF-κB by anti-oxygen free radicals.