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黑果枸杞(Lycium ruthenicum Murr.)和宁夏枸杞(Lycium barbarum L.)系茄科枸杞属植物,具有非常重要的药用价值。本研究通过挖掘黑果枸杞和宁夏枸杞的转录组数据克隆获得其开花关键基因FT(Flowering Locus T),分别命名为Lr FT和Lb FT,通过生物信息学方法分析两个蛋白质的氨基酸序列、功能域及其系统发育关系等;并通过实时定量PCR分析其在两种枸杞不同组织中的表达模式。研究结果表明:Lr FT和Lb FT均编码一个由173个氨基酸组成的蛋白质。蛋白序列分析表明:Lr FT和Lb FT序列相似度达98%,具有保守的PEBP(phosphatidylethanolamine-binding protein)结构域。系统进化分析表明:Lr FT和Lb FT与茄科植物番茄、烟草和马铃薯的FT亲缘关系较近。基因表达结果表明:Lr FT和Lb FT主要在叶片中表达,且在两种枸杞的叶片中表达量有一定的差异。本研究为今后黑果枸杞和宁夏枸杞开花相关功能基因的验证及分子育种奠定了一定的基础。
Lycium ruthenicum Murr. And Lycium barbarum L. are the key wolfberry genus Lycium, which has very important medicinal value. In this study, flowering locus FT (Flowering Locus T) was cloned by transcriptome data mining of Lycium barbarum and Lycium barbarum, and named as Lr FT and Lb FT respectively. The amino acid sequences and functions of the two proteins were analyzed by bioinformatics Domain and its phylogenetic relationship, etc .; and through real-time quantitative PCR analysis of its expression in two different tissues of Lycium. The results showed that both Lr FT and Lb FT encoded a 173 amino acid protein. Protein sequence analysis showed that the sequence similarity of Lr FT and Lb FT was 98%, and the conserved PEBP (phosphatidylethanolamine-binding protein) domain was found. Phylogenetic analysis showed that Lr FT and Lb FT are closely related to FT of Solanaceae tomato, tobacco and potato. The results of gene expression showed that Lr FT and Lb FT were mainly expressed in leaves, and there were some differences in the expression of Lr FT and Lb FT in the leaves of two kinds of Lycium barbarum. This study laid the foundation for the future verification of flowering-related functional genes and molecular breeding of Lycium barbarum and Ningxia wolfberry.