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本文报道对15例人正常肝组织微体的立体定量研究,以及过氧化氢酶(CAT酶)、葡萄糖-6-磷酸酶(G-6-P酶)、焦磷酸硫胺素酶(TPP酶)、胞嘧啶单核苷酸酶(CMP酶)细胞化学反应的观察。人肝细胞的微体大多呈椭圆形,内含均匀细颗粒状物,单层膜包绕,在胞浆中散在。微体的体密度为1.33±0.38％,数密度为9.76±1.63个/100μm~3,线粒体/微体的数量比为6.22±1.17。酶细胞化学反应的研究发现,内质网腔与微体未见相通,与微体相连的类似滑面内质网的膜结构,呈G-6-P酶细胞化学反应阴性,表明此膜结构不是内质网,可能属于微体的膜系统。高尔基体与微体在结构上未观察到移行关系。而一些微体出现分裂、芽生的现象。此结果表明,新的微体可能由细胞中原有的微体分裂产生。
In this paper, we reported the stereological quantification of 15 human normal liver microdomains and the expression of catalase (CAT), glucose-6-phosphatase (G-6-P), pyrophosphate pyrophosphate (TPP) ), Cytosine mononucleotidase (CMP enzyme) cytochemical reaction observed. Human hepatocytes are mostly oval-shaped micro-body, containing uniform fine particles, surrounded by a single membrane, scattered in the cytoplasm. The body density of the microsome was 1.33 ± 0.38%, the number density was 9.76 ± 1.63 / 100μm ~ 3, the number ratio of mitochondria to the microsome was 6.22 ± 1.17. The study of enzymatic cell chemistry found that the endoplasmic reticulum lumen did not communicate with the microtubules, and the membrane structure similar to the glomerular endoplasmic reticulum that was connected with the microtubules showed a negative G-6-P enzyme chemical reaction, indicating that the membrane structure Not the endoplasmic reticulum, which may belong to the membrane system of the microdomains. No structural relationship between the Golgi apparatus and the microsomes was observed. And some micro-body split, sprouting phenomenon. This result shows that the new micro-body may be generated by the original cell division in the microdissection.